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باقر افتخاری سیس

باقر افتخاری سیس

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده علوم پایه
نشانی:
تلفن: +۹۸۴۱۳۷۲۷۸۹۰۰-Int.۵۱۲

مشخصات پژوهش

عنوان
A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Breast cancer; graphene oxide; 185delAG mutation; nano-biosensor; fluorescent
سال
2016
مجله CANCER INVESTIGATION
شناسه DOI
پژوهشگران Bagher Eftekhari-Sis ، Sajede Karaminejad ، Farrokh Karimi

چکیده

Amethod was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5 terminal in the presence of cDNA or mDNA was applied.