May 19, 2024
University of Maragheh
فارسی
Bagher Eftekhari-Sis

Bagher Eftekhari-Sis

Academic rank: Professor
Address: Department of Chemistry, University of Maragheh, P.O.Box 55181-83111, Maragheh, Iran
Education: PhD. in Organic Chemistry
Phone: +984137278900-Int.512
Faculty: Faculty of Basic Sciences
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Research

Title
A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer
Type Article
Keywords
Breast cancer; graphene oxide; 185delAG mutation; nano-biosensor; fluorescent
Year
2016
Journal CANCER INVESTIGATION
DOI 10.1080/07357907.2016.1227444
Researchers Bagher Eftekhari-Sis، Sajede Karaminejad، Farrokh Karimi

Abstract

Amethod was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5 terminal in the presence of cDNA or mDNA was applied.
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