In order to investigate the effects of different humidity conditions with the use of different fungi on the changes in the defense system of the Danai thyme plant and also the amount of essential oil of this plant, a factorial experiment was conducted in the form of a randomized complete block design with 12 treatments and in 3 replications in a research greenhouse. Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Maragheh University. The fungal treatments used in this experiment are: no inoculation, P. indica fungus, Trichoderma fungus, P. indica and Trichoderma fungal combination, as well as applied stresses, moisture stress were 90, 65 and 45% of the agricultural capacity. The results of analysis of variance obtained from the data showed that the interaction treatment of mushroom application and drought stress on stem height, chlorophyll a and b, carotenoid was significant at the probability level of 1%. The use of Indica mushroom, Trichoderma mushroom, and the mixture of Indica and Trichoderma fungi in 45% humidity conditions increased the stem height by 14.32, 33.68, and 56.25%, respectively, compared to the treatment without mushroom inoculation in 45% moisture stress conditions. Based on the results of the comparison table of the average amount of chlorophyll a and b, carotenoid has the lowest activity under the condition of no mushroom application and 45% moisture stress, and has the highest activity under the treatment of P. indica and Trichoderma mixture under 90% moisture condition. to be Moisture stress reduces the growth rate by reducing the absorption of nutrients and reducing the efficiency of photosynthesis. Trichoderma fungi have the ability to stimulate growth and induce plant resistance, and this type of symbiotic fungus has high reproductive ability. Moisture stress has the greatest effect on the light part of photosynthesis. Moisture stress causes oxidative stress in chloroplasts and creates various types of activ