Drought stress and zinc deficiency have always been considered as problems affecting the yield of cereals, especially wheat. In order to influence of zinc application and and its internal amount on growth and defense system in different moisture conditions, a factorial research was conducted based on a completely randomized design at Ardabil University and its biochemical parameters were measured at Maragheh University in 2017. Factors studied included biofortified seeds, application of zinc and drought stress. The results of this study showed that the three-way interaction on stem height, leaf area, chlorophyll, superoxide dismutase activity, Cu/Zn-SOD isozyme, ascorbate peroxidase, catalase and hydrogen peroxide, malondialdehyde, proline was significant at 1 and 5% probability levels. According to the mean comparisons, the highest activity of superoxide dismutase, Cu/Zn-SOD isozyme, ascorbate peroxidase and catalase in 30% of field capacity was recorded in the treatment of increased seeds by soil method. The result can be seen in modulating the amount of hydrogen peroxide and malondialdehyde in this treatment. Also, based on these results, the amount of chlorophyll, leaf area and plant height in this treatment showed a significant increase compared to the treatment of control seed application in irrigation conditions of 30% of field capacity. As a final result, it can be acknowledged that the application of soil-biofortified seeds in the Registered Seed fields along with the application of 25 kg/ha of sulfate zinc in the main fields in different moisture conditions, can be introduced as a suitable treatment for field research.