In order to γ_aminobutyric acid spraying on Thyme enzymatic antioxidant change under deficit irrigation treatment, this experiment was carried out as factorial experiment conducted based on a randomized complete block desing with three replications in the research greenhouse of the Department of Plant Production and Geneteics, Maraghe University. The main factor included levels of deficit irrigation (30%, 60%, 90% field capacity) and sub factors included no spraying of GABA. 25 mM GABA, 50 mM GABA and 75 mM GABA. According to the results, the activity of superoxide dismutase and proline was effected by the intraction of water stress × foliar spraying. According to the results, the activity of the enzyme of the superoxide of demotase and proline was affected by the interaction of dehydration stress in discovering. The results of mean comparison showed that the occurrence of midel intensity stress (60 % FC) the activity of superoxide dismutase enzyme increased and then at high stress intensity (30 % FC) decreased. GABA also increased the activity of this enzyme activity, and the highest level of superoxide dismutase enzyme was obtained from the 25mm GABA at 60 % FC. Also proline levels increased with the intensity of stress. In addition, the highest and lowest proline levels were obtained respectively from the use of 25 mm of GABA in the stress of 60 % FC and the non -use of GABA in 90 FC. At 60 % FC, GABA spraying compared to non-spraying of GABA increased proline by 60 %.