In order to study on physiological response and selection of cold tolerant wild chickpea genotypes, an exprimen was conducted with 24 annual wild Cicer species at Maragheh agriculture research stations during two crop seasons under cold stress. In the first year wild genotypes along with ANA and ILC533, as tolerant and susceptible genotypes, were planted in the field in autumn in a randomized complete block design with two replications. The results of field experiment showed that of the 24 genotypes studied, 3, 11 and 10 genotypes were identified as cold susceptible, cold tolerant and cold resistance, respectivly. In the second year, the experiment was performed with two factors incloding; cold stress 22°C, 4°C and -4°C and ILWC109 and ILWC120 along with ANA and ILC533 genotypes in the form of the factorial arrangement in a completely randomized design with two replications in the growth chamber. The results showed that low temperature stress (-4°C) cuased significant decrease chlorophyll (a, b), cartenoid and some of antioxidant enzymes, especially in the control genotype (ILC533). During stress (22°C, 4°C and -4°C), Fv/Fm of chickpea genotypes was 0.79, 0.64 and 0.51 respectively, that represent the effect low temperature stress to Fv/Fm in all treatments especially -4°C. In addition, the results showed that in low temperature stress, ILWC109 genotype had high yield Fv/Fm than to other genotypes. In general the using of Fv/Fm, chloropyll content and caratenoied leaf parameters is recommended for screening and evaluating of chickpea genotypes in under low temperature stress conditions at fields.