Spring cultivation of lentil, confront drought stress and leads yield decrease in cold regions. In order to study effect of nano ZnSO4 and Fe2O3 spray on yield and antioxidant system in lentil, a split split plot design based on complete block design with three replications done at dry land agricultural research institute- Maraghe at 2019-2020. Respectivively Different irrigation levels, cultivars (Kimia and Bilesavar) and doses of spraying nanoparticles (no spraying-0.5% nano ZnSO4- 1% nano ZnSO4- 0.5% ZnSO4- 1% ZnSO4- 0.5% nano Fe2O3- 1% nano Fe2O3-0.5% Fe2O3- 1% Fe2O3 ) were considered as main, sub and sub-sub-plot. Results showed that, the effect of stress on RWC, yield, CMS was significant at 1% level. Effect of cultivar on RWC1-2 and CMS was significant respectively at 5 and 10% level. Effect of spraying nano particles was significant on biomass, NDVI1, canopy tempreture, RWC1 and yield respectively at 5, 10, 5, 10 and 1% level. Interaction of strees × spraying nano particles on NDVI1, 2, 3 was significant at 5, 10 and 5% level. Cultivar × spraying nano particles interaction was significant at 10% level on NDVI3. The interaction of stress × cultivar × spraying nanoparticles was significant on CMS at 5% level. The effect of stress was significant for chlorophyll a, cartenoides, activity of Superoxid dismutase, malondialdehyde, glotathion peroxidase, ascorbat peroxidase and catalase at 1% level and for chlorphyll b, prolin at 5% and H2O2 at 10% level. Effect of cultivar was significant for chlorophyll a, b, cartenoids, prolin, activity of Superoxid dismutase, glotathion peroxidase, ascorbat peroxidase, catalase and content of malondialdehyde at 1% and for H2O2 at 5% level. Effect of spraying nanoparticles were significant for chlorophyll a, b, H2O2, malondialdehyde, glotathion peroxidase, ascorbat peroxidase and catalase at 1% level. Interaction of stress and spraying was significant for chlorophyll a, b, H2O2, malondialdehyde, ascorbat peroxidase and catalase a