Due to the fact that wheat (Tritium aestivum L.) is often cultivated in drylands, it is necessary to know the effects of drought stress on its physiology in order to develop cultivars resistant to this stress.In this research, firstly, the top cultivars and lines of the Dryland Agricultural Research Institute (DARI) were examined during three crop years (2017-2020) in the form of a simple lattice design. Genotypes No. 4 and 40 were selected as superior cultivars under stress and genotypes No. 30 and 33 under supplementary irrigation conditions.Based on the comparison of averages, the highest activity of antioxidant enzymes including ascorbate peroxidase, superoxide dismutase and catalase was recorded in genotype number 4 under rainfed conditions and the lowest activity of these enzymes was recorded in genotype number 40 under supplementary irrigation conditions.