Savory is a medicinal plant known for its aromatic properties and therapeutic benefits. The chemical composition of the plant can vary based on the species and the climate of its growing area. One of the environmental factors that affect the growth and development of plants is water stress. Water stress plays a crucial role in reducing the yield of plants worldwide. this research aims to investigate the level of lipid peroxidation in Savory under the influence of water stress and ascorbic acid spraying at various concentrations. This study was conducted in the year 1400 at the research greenhouse of Maragheh University as a factorial design in a randomized complete block design with three replications. The investigated factors include water stress at three levels: 90, 60, and 30% of field capacity (FC) and spraying of ascorbic acid at three levels: 0, 1, and 2 mM. The results obtained from this research showed that an increase in water stress intensity led to a significant increase in lipid peroxidation. Additionally, the spraying of ascorbic acid, particularly in high concentrations, resulted in a significant decrease in the level of MDA. Also, according to the results, the activity of the guaiacol peroxidase (GPX) enzyme initially increased and then decreased with the increase in stress intensity. Furthermore, the spraying of ascorbic acid, especially at a concentration of 2 mM, significantly enhanced the activity of this enzyme.