احمد آقایی

In order to investigation of the effect of plant growth regulators Indole-3-Acetic Acid (IAA) and Kinetin (Kin) on callus induction and regeneration of Mooseer explants under in vitro culture condition, a factorial experiment in a completely randomized design with four replications were conducted. The effect of different concentrations of above mentioned plant hormones in four levels (0, 0.5, 1, and 2 mg L-1) on callus induction and regeneration of stem disc explants of Mooseer in Murashige and Skoog (MS) medium were investigated. Changes in growth parameters in MS medium were observed from 21th day. Results showed that in media containing IAA and without Kin, no bulblet was generated and explants were produced callus. In treatments containing 0 and 0.5 mg L-1 IAA plus 2 mg L-1 Kin, regeneration and callus formation were observed simultaneously. The highest growth of bulblets were produced in treatments containing 1 and 2 mg L-1 Kin plus 0 and 1 mg L-1 IAA and the lowest one were observed in control explants. The best hormonal proportion in order to callus induction was 2 mg L-1 IAA without Kin.