Nanoparticles with magnetic characteristics have a vast biomedical application, which can be attributed to their controllable properties and vast functions [1]. Magnetic nanoparticles (MNPs) have various applications in medical diagnosis and treatment such as drug delivery and targeted imaging. Precise assembly of MNPs using polymeric media can promote their biomedical applications due to the possible design of media by changing chemical structure, molecular weight, electric charge, and other macromolecular characteristics of polymers [2]. Different polymeric materials such as poly (N-isopropylacrylamide) (PNIPAM), poly(acrylic acid) (PAA), poly(vinyl alcohol) (PVA), poly(styrene sulfonic acid) (PSS), polyethylene glycol, chitosan, poly(methyl methacrylate) (PMMA), polyurethane (PU), poly(vinyl pyrrolidone) (PVP), polylactic acid (PLA), poly (N-vinylcaprolactam), polyaniline (PANI), and poly(vinyl chloride) (PVC) have been utilized as media for directed assembly of MNPs. This chapter aims to review polymer-mediated MNPs for biomedical applications.