Background and Aim: Wheat has a special role in provid- ing the required energy and protein of people in the de- veloping countries like Iran. Wheat is confronted with nutri- tional limitations such as little zinc (Zn) and low biological value of protein, which results in hidden hunger and mal- nutrition. Methods: This research was conducted to study the effect of Zn bio-fortification on wheat protein and amino acids contents in randomized complete block design with three replications. The treatments were non-using Zn sulfate (control and/or Zn deficient stress) and the use of Zn sulfate (spraying 0.5 g/L at stem elongation and 2 g/L at grain fill- ing stages). Results: The results indicated that in Zn treatment, the amino acids contents, total Lysine and Threonine were in- creased that resulted in higher grain protein content and Zn concentration in compared to Zn deficient stress. Conclusion: It can be concluded that Zn foliar application could alleviate malnutrition by increasing grain Zn content and protein under Zn deficient stress. Therefore, fertilizer strategy (e.g., agronomic bio-fortification) appears as short- term solution to alleviate malnutrition problem.
