The aim of this project is to investigate the method of using a common buffer to determine the degree of sta- bilization and secretion of two drug molecules that have been analyzed in vitro. First, magnetic nanoparticles were synthesized and their structure was identified by instruments such as XPS (X-ray photoelectron spectros- copy) and FT-IR (Fourier transform infrared spectroscopy). The main purpose of this study was to investigate the stabilization and release of methotrexate on the surface of magnetic nanoparticles. The two temperatures were 37 and 25◦, respectively. After reaction with the biomolecules, the adsorption rate for both drug molecules was about 60–80. PBS buffer was also used for diffusion of biomolecules and the results were analyzed by spectro- photometer analysis. With these results, the adsorption of cysteine and MTX was more than 60% and its release rate in MNPS-IHSPN was up to 90%, which means that high-strength stabilization and release by magnetic nanoparticles under external magnetic field and in vitro confirmed. The result of this project for the exchange of drugs by the surface of magnetic nanoparticles to repair damaged cells in the body of living organisms can be generalized.
