New mononuclear Ru(II)-thienyl-terpyridine complex [Ru(thienyl-tpy)(bpy)Cl]Cl (where thienyl-tpy = 4’-(2-thienyl)-2,2’:6’,2’’-terpyridine, bpy = 2,2’-bipyridine) has been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV−vis, 1H and 13C NMR) and mass spectrometric technique (ESI Q-TOF MS). Furthermore, the interactions with CT DNA were performed both in the absence and presence of biocompatible ionic liquids 1-amino-2-propanol-lactate (IL1), 1-amino-2-propanol-oxalate (IL2) and 1-amino-2-propanol-citrate (IL3)