In this study synergistic effect of the biosurfactants saponin and rhamnolipid on the removal of the heavy metals such as vanadium, nickel and chromium from contaminated soil was investigated. Washing solutions were prepared by using different concentrations of two biosurfactants. In this work, the optimum HLB value of the extraction process of about 10.5 was obtained for the mixture of 62.5% rhamnolipid and 37.5% saponin. According to our results, at the optimal HLB value, a maximum removal was obtained for Ni of 87%, Cr of 71% and Va of 70%. Our results also showed that the efficiency of heavy metals decreased with increasing the pH value of system. The optimum pH value of heavy metals removal was 5. According to the results of this research saponin and rhamnolipid have the synergistic effect on the heavy metals removal and it seems to be a good alternative to chemical surfactants for environmental applications.
