In this paper, a reliability-based approach has been implemented to develop seismic analytical fragility curves of highway bridges. A typical bridge class of the Central and South-eastern United States (CSUS) region was selected. Detailed finite element modelling is presented and Incremental Dynamic Analysis (IDA) is used to capture the behavior of the bridge from linear to nonlinear behavior. Bayesian linear regression method is used to define the demand model. A reliability approach is implemented to generate the analytical fragility curves and the proposed approach is compared with the conventional fragility analysis procedure.