Deep eutectic solvents (DESs) and ionic liquids have been considered as organic solvents and applied in numerous chemical procedures. DESs usually are produced by combining two or three organic components. DES can be formed in a mixture by combining the hydrogen bond donors such as urea, polyols, and carboxylic acids with the hydrogen bond acceptors. In the present study, deep eutectic solvents as green solvents, and extraction method known as Headspace-solvent microextraction were used. Deep eutectic solvents hydrodistillation single drop microextraction (DES-HS-SDME) was used for extracting the essential oil compounds using DESs as extraction solvents prepared by combining the choline chloride and p-chlorophenol. They were used to extract the volatile compounds from Satureja hortensis L. leaves. To avoid the traditional and time-consuming hydrodistillation, the volatile components in Satureja hortensis L.’s aerial parts were analyzed through a simple distillation using headspace single-drop microextraction along with gas chromatography. According to the experimental findings, DESs-HS-SDME is a rapid, low-cost, simple, and reliable technique to determine the volatile fractions in Satureja hortensis L.
