: In the present work, the Headspace-Solid Phase MicroExtraction (HS-SDME) process was established utilizing a novel version of Deep-Eutectic-Solvents (DESs) for extracting and preconcentrating essential oils in plants followed by GC-FID determination. DESs seem to be a costeffective and attractive alternative for the usage of ionic solvents in biotransformation. DESs are usually developed through gentle warming and stirring of two (low-cost and bio-based) salts. DESs have several superiorities over ionic liquids such as their ease of preparation, low production cost, and permit for large-scale applications. HS-SDME was made in the current research in order to extract volatile compounds by the use of Deep Eutectic Solvents (DESs) serving as extraction solvents. HS-SDME was constructed as a solvent-minimized extraction method. However, there are a rare number of studies investigating the Deep Eutectic Solvents (DESs) applications to the HS-SDME of bioactive compounds. Deep eutectic solvents, created by mixing choline chloride (ChCl) and p-Chlorophenol at varying ratios were utilized for extracting essential oils from Echinophora platyloba DC via HS-SDME in the present study. Afterward, headspace single-drop micro-extraction (HS–SDME) was conducted, being connected to gas chromatography. HS-SDME is a quick and simple method in comparison with heat reflux extraction. In addition, it is possible to use DESs in HS-SDME for extracting various volatile compounds.
