Wound dressings are the main barrier between the wound area and the out environment. Here we design a novel electrospun nanofibers (NFs) based on polyurethane/polyacrylic acid/poloxamer (PU/PAA/POLO) as a biocompatible wound dressing. PU/PAA NFs with different weight concentration of POLO (5%, 10%, and 15%) were produced by electrospinning and the chemical structure, thermal stability, surface morphology, porosity, cytocompatibility, swelling and degradation ability, hydrophibicity and mechanical properties were investigated. Based on the obtained results, the PU/PAA/POLO fibers showed a uniform and beads-free morphology with acceptable mechanical characteristics. By increasing the weight concentration of POLO added to the NF structure, an increase in the diameter of NFs (486.47 ± 251.838 nm), swelling ratio (about 3%), wettability (51.59°), and degradation degree (76%) were observed. Moreover, with increasing the diameter of NFs, the porosity (65.28 ± 0.9%), and WVTR (270.89 ± 3.01 g/m2.24 h) of NFs were significantly (P < 0.05) reduced. The MTT assay illustrated that produced NFs were non-toxic to the cells and the cell adhesion study showed that the developed NFs can form a good candidate platform for cell adhesion and proliferation. Based on these findings, it is expected that the produced PU/PAA/POLO NFs to be used as a wound dressing and accelerate the wound healing process.
