Chemiluminescence (CL) reactions are chemical reactions in which the energy of reaction is
released in the form of light. In recent decades, CL- based analytical methods have received
extensive attention due to their advantages such as high sensitivity,wide liner range, simplicity
and inexpensive equipment [1]. Graphene oxide (GO) is a material in the form of sheet with
atom thickness that can disperse in water [2]. GO can intensify the light emission from the CL
reaction of luminol - oxygen. This phenomenon is due to the ability of GO in production of
reactive oxygen species [3]. Dobutamine prescribed for heart disease treatment and its
measurement in the pharmaceutical industry is very important from the quality of manufactured
drugs point of view. In this work, a new, simple and inexpensive method was developed to
measure dobutamine. The method was based on inhibitory effect of dobutamine on the GOluminol-
O2 CL reaction. GO was synthesized by improved Hummers method. The synthesized
GO was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD),
ultra violet absorption (UV-Vis) and fourier transform infrared spectrometry (FTIR).. The effect
of concentrations of GO, NaOH and luminol were on CL intensity was investigate and
optimized. Under the optimum conditions, a liner relationship was obtained between CL
intensity and concentration of dobutamine in the range of 3×10-7-5×10-6mol L-1. The detection
limit of this method 2.4 × 10-7 mol L-1 and its relative standard deviation (RSD) for nine replicate
determinations of 2 × 10-6 mol L-1 dobutamine was 1.2%