In the present study, a simple, rapid, sensitive, and environmentally friendly method was proposed for the determination of dobutamine hydrochloride (Dbn.HCl). The method is based on the inhibition of the chemiluminescence (CL) arising from the reaction of luminol and graphene oxide (GO) in a strongly alkaline medium by Dbn.HCl. GO was synthesized and fully characterized by various spectroscopic methods, including TEM and SEM. The mechanisms of the generation and inhibition of CL were also investigated and discussed. The experimental conditions were optimized and under optimum conditions, the method showed a linear response for Dbn.HCl over the concentration range from 3.0 107 to 5.0 106 mol L1. The correlation coefficient (r2) of the calibration line was 0.9972, and the relative standard deviation (RSD) of the method obtained by nine replicate determinations of 2.0 106 mol L1 Dbn.HCl was equal to 1.2%. The method was successfully used for the determination of Dbn.HCl in various real samples.
