importance of this antibiotic, several methods for the determination of isoniazid have been reported. In general, chemiluminescence (CL) is the emission of electromagnetic radiation from a chemical reaction which is usually exothermic [2]. Hao et al [3]. reported that graphene oxide (GO) can enhance the intensity of light emission CL reaction luminol – O2. This catalytic activity was due the fact that the GO can react with the dissolved oxygen and produce O2 -•, oxidizing luminol to produce CL emission. In this work, GO were used for enhancing of luminol– O2 CL reaction. To this aim, we synthesized GO by using improved hummer method. Then, the prepared GO were characterized by ultraviolet and visible (UV-Vis) spectroscopy, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and also transmission electron microscopy (TEM). Then, we found that isoniazid drug were capable of enhancement CL emission of GO enhanced luminol – O2. Based on this finding, experimental conditions such as concentrations of luminol, sodium hydroxide and GO were examined and optimized. The proposed method exhibited good performance and its linear range was from 1 × 10-7 – 1 × 10-4 M. Moreover, the relative standard deviation and limit of detection for the method for isoniazid were 3.38% (n = 5) and 7.9 × 10-8 M, respectively. The method was applied to the determination of isoniazid in pharmaceutical formulations and water samples.
