In this work, it was found that pramipexole (PPX) could decrease the intensity and rate of the CuO NP-catalyzed HCO3–H2O2 CL system. Based on these findings, the CuO NPs–HCO3–H2O2 CL system was used for the determination of PPX. With this aim, the experimental parameters influencing the CL reaction were optimized to achieve the highest effect of PPX on the CL system. After optimization, two calibration graphs including CL intensity versus PPX concentration and time of appearance of the maximum CL intensity (Tmax) versus PPX concentration were plotted. The linear range for the calibration graph based on CL intensity versus PPX concentration was 1.0 106 to 2.0 105 mol L1 with the correlation coefficient of the calibration line being r2 ¼ 0.9938. The limit of detection (LOD) was equal to 7.9 107 mol L1 and the relative standard deviation of the method with respect to 1.0 105 mol L1 PPX was equal to 4.7% (n ¼ 10). The linear range for the calibration graph based on Tmax versus PPX concentration was 7.0 107 to 5.0 104 mol L1 with the correlation coefficient of the calibration line being r2 ¼ 0.9983. The LOD was equal to 3.4 107 mol L1 and the relative standard deviation of the method with respect to 1.0 105 mol L1 PPX was equal to 3.19% (n ¼ 10). The utilized CuO NPs were prepared by exploiting a quick precipitation technique and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and dynamic light scattering (DLS) techniques. The developed method was successfully applied to the determination of PPX in pharmaceutical formulations and spiked tap water.
