In this paper, a NiO hollow microspheres-luminol-dissolved oxygen chemiluminescence (CL) system was proposed for determination of isoniazid in tablet and serum samples. The NiO nanoparticles (NiO-NPs) were prepared by a simple hydrothermal method which were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR). The NiO-NPs have a catalytic effect on the luminol- dissolved oxygen CL system for measuring isoniazid. The effect of important parameters on determining method efficiency, such as concentration of NaOH, luminol and nanoparticles were investigated. At the optimum conditions, the calibration curve for isoniazid was obtained in the range of 8×10-7 to 1×10-4 M (R2=0.99). The detection limit of the method was obtained 2×10-7 M. In addition, the proposed method was successfully used to determination of isoniazid commercial tablets and human serum as real samples. The acceptable relative standard deviation (RSD) was obtained for the proposed CL method (2.63%, n=10).
