Chemiluminescence (CL) refers to the phenomenon of light emission resulting from the electronic excitation of species that arises during an exothermic chemical reaction. Researchers have extensively utilized CL reactions to develop novel, effective analytical techniques, featuring high sensitivity, broad linear range, cost-effectiveness in equipment, and convenient operability [1]. This study presents a CL method employing flower-like CoSe2 nanostructures (NSs) catalyzed luminol–O2 CL reaction for the determination of uric acid (UA) and dobutamine HCl (DBU HCl). The CL method is based on the phenomenon of quenching induced by the analytes understudy in the CL reaction. To attain the most quenching efficacy of the analytes in the CL reaction, the concentrations of reactants comprising luminol, NaOH, and flower-like CoSe2 NSs were optimized accordingly. The calibration curves demonstrated exceptional linearity within the concentration range from 1.00 10-5 to 1.00 10-4 mol L-1 and 1.00 10-6 to 1.00 10-5 mol L-1 for UA and DBU HCl, respectively. This study synthesized CoSe2 NSs using a facile hydrothermal approach, and then the synthesized flower-like CoSe2 NSs were subjected to a comprehensive characterization utilizing a range of spectroscopic methods, including scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM) and Fourier-transform infrared (FT-IR).
