Investigation of genotypes for their yield stability under various environments is important issue in plant breeding programs. Yield stability of 16 barley (Hordeum vulgar L.) genotypes was tested across four locations and three years. The analysis of variance showed that genotype (G), environment (E), and their interaction (G × E) were significant for barley yield. The presence of a large G × E interaction magnitude indicates a significant problem of related to phenotypic expression and makes it difficult for decision making in selection. According to means of environments and total general mean yield (3446.9 kg ha-1), test environments are grouped as H (six high yield) and L (five low yield). The identified most stable genotypes with static stability concept and based on the minimum spanning tree plots and centroid distances were G5 with 3165.4 kg ha-1 and G16 with 3191.7 kg ha-1, and therefore could be recommended for unfavorable or poor conditions. Also, genotypes G5 (3165.4 kg ha-1), G13 (3119.1 kg ha-1) and G16 (3191.7 kg ha1 ) were located several times in the vertex positions of high cycles according to the principal coordinates analysis. Therefore, genotypes G5 and G16 were found to be the most stable genotypes in both favorable and the favorable conditions and are recommended for commercial release. The principal coordinates analysis provided useful and interesting way of investigating GE interaction of barley genotypes. Finally, the results of principal coordinates analysis in general confirmed the breeding value of the genotypes, obtained on the basis of the yield stability evaluation.
