Evaluation of sulphur nanoparticles effect on antioxidant agents of Mooseer Parisa Fathi Rezaei1, Mahsa Moghaddam Heidari2 1Department of Biology, University of Maragheh, Maragheh, Iran 2Department of Biology, Science and Research Branch of Islamic Azad University, Tehran, Iran Introduction The sulfur as an important macronutrient in plant biology is a vital component of housekeeping biomolecules and of compounds involved in defence against biotic and abiotic stresses in all or some plants. The nanoparticles interact with plants causing many morphological and physiological changes depending on their chemical composition, size, surface covering, reactivity and concentration. The phenolic compounds are the most abundant secondary metabolites in plants and have wide range of biochemical and pharmacological effects such as; antioxidant, anti-inflammatory, antimicrobial, anticancer, inhibition of platelet aggregation, prevention of cardiovascular disease and a general anti-aging activities. Flavonoids are one of the largest and most abundant groups of plant secondary metabolites. Allium hirtifolium Boiss with the local name of Mooseer (Alliaceae) is an Iranian native edible plant, has been widely used as medicine and condiment predates. In this experiment we synthesized and evaluated the effects of sulphur nanoparticles (SNPs) on the total phenol and flavonoid contents of the Mooseer explants in comparison to the sulphur. Methods Sulfur nanoparticles were synthesized through novel water-in-oil microemulsion system. The bulbs of Mooseer were sterilized and stem disc parts were cultured in MS medium supplemented with various concentration of sulfur nanoparticles and sulfur (0, 25, 50, 75 and 100 ppm). After one month samples were collected. The total polyphenol content of the samples was determined by Folin-Ciocalteu colorimetric assay, using gallic acid as standard and the absorbance of the samples was measured at 725 nm using spectrophotometer. The total flavonoid content
