رضا معصومی جهاندیزی

Background: TEM beta lactamase gene is one of the important plasmid genes in Enterobacteriaceae which is the cause of over 90% of Escherichia coli isolates resistance to beta lactam antibiotics. The aim of this study was to detect the prevalence of antibiotic resistance and TEM gene. Materials and Methods: During 6 months (June to November 2015) 266 clinical isolates of E.coli were collected from laboratories in Bonab County. Phenotypic screening and confirmation tests for extended spectrum beta lactamases (ESBLs) were carried out using disk diffusion (Kirby Bauer) method. All of the ESBL producing isolates were tested by PCR using specific primers. Results: our results showed that, the maximum resistance was seen for ampicillin (67.3 %) and the maximum sensitivity was seen for imipenem (92.5%). In this study 45 % isolates were multidrug resistance, which showed at least resistance for three antibiotics. Out of 154 isolates, 58 (37.7%) cases were ESBL producers which 65.51% of isolates contained TEM gene. Conclusion: This study showed that, TEM gene encodes over 50% of ESBLs in E.coli. Therefore, we recommend detection of this gene as a routine bacteriologic procedure in management of the nosocomial infections caused by enteric bacteria.