Abstract: Many studies have shown that miRNAs can silent the genes involved in the growth and invasion of cancer cells. However, the exact effect of miRNAs on the expression of Snail-1 CXCR4 and miRNA-143 genes is not entirely clear in breast cancer cells. Therefore, the present study investigated the effects of siRNA on Snail-1, CXCR4 and miRNA-143 in breast cancer cells in vitro. In this experimental-laboratory study, MDA-MB-468 breast cancer cells were transfected by the specific siRNA of the Snail-1 gene. The expression levels of Snail-1 CXCR4 and miR-143 genes were evaluated by qRT-PCR. Cell proliferation was assessed by trypan blue test. Data were analyzed using student’s t-test. Transfecting the MDA-MB-468 breast cancer cells by specific siRNA significantly decreased the expression level of Snail-1 gene CXCR4 and significantly increased the expression of miR-143 (p < 0.05). The results of this study showed that knocking down of diagnostic markers of breast cancer cells by specific siRNA can successfully reduce the proliferation and invasion of breast cancer cells.
