Pectinases are one of the most important industrial enzymes and hydrolyze pectin to galacturonic acid. Pectin is one of the main polysaccharides in plant cell wall. Pectinases have a large number of applications in food industries and biomass conversion. Various methods are used for pectinase purification from microbial sources including precipitation and chromatography. Nowadays many studies focused on purification and immobilization of enzymes on magnetic beads. In this investigation purification of Piriformospora indica pectinase on kappa carrageenan-chitosan nano magnetics was investigated. P. indica was cultured on YPG medium supplemented with pectin. The slurry was filtered by centrifugation at 10,000 rpm at 4 ºC for 15 min. Later the supernatant was incubated with kappa carrageenan-chitosan nano magnetics at 4 ºC overnight with continues shaking. The enzyme was desorbed from nano magnetics by phosphate buffer. The protein content of samples was determined by Bradford assay. Based on the results, the protein content of the solutions treated with kappa carrageenan-chitosan nano magnetics was elevated significantly in comparison to untreated solutions. In conclusion, kappa carrageenan-chitosan nano magnetics could open new insights for purification of pectinase and the optimization of its immobilization on magnetic beads is running in our group.
