The pollution of natural water resources with agricultural pesticides is of great environmental concern. Glyphosate is a widely wed herbicide that belongs to organophosphate chemicals. The presence of this contaminant in the water greats serious problems for living organisms. In this work, Zn-Chitosan/ZnO nanocomposite was prepared and employed as an adsorbent for selective and efficient removal of glyphosate from natural water. The adsorption data for removal of glyphosate by Zn-Chitosan/ZnO nanocomposite were fitted to many isotherm models. The effect of the initial pH value on the adsorption was also investigated. It was found that the selectivity of the modified adsorbent was stadied in the presence of natural water common anions. The results showed that the modified nanocomposite has higher capacity and more desired selectivity for glyphosate adsorption at neutral pH values.