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Title
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A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer
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Type
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JournalPaper
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Keywords
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Breast cancer; graphene oxide; 185delAG mutation; nano-biosensor; fluorescent
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Abstract
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Amethod was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5 terminal in the presence of cDNA or mDNA was applied.
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Researchers
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Farrokh Karimi (Third Researcher), Sajede Karaminejad (Second Researcher), Bagher Eftekhari-Sis (First Researcher)
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