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Title
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Production of laccase by recombinant Yarrowia lipolytica from molasses: Bioprocess development using statistical modeling and increase productivity in shake-flask and bioreactor cultures.
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Type
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JournalPaper
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Keywords
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Yarrowia lipolytica, Laccase, Molasses, optimization, Bioreactor
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Abstract
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Laccases are used in numerous applications, from green degradation of various xenobiotic compounds, waste detoxification, textile dye bleaching, and delignification of lignocellulose materials to biofuel production. In this study, the recombinant Yarrowia lipolytica YL4 strain carrying the white-rot fungusTrametes versicolorlaccaseIIIbgenewas used for laccase production from beet molasses as an agro-industrial residue. Response surface methodology was used to statistical optimization of the production of laccase byY. lipolytica using an industrial medium containing molasses which allows a six times increase in laccase activity compared to primary medium contains glucose after 144 h. In bioreactor cultivation after 48 h, laccase production reached to 3.7- and 22.5-fold more than optimized and primary media in shake-flask cultures, respectively. Laccase productivity in bioreactor (0.0937 U/h) was higher than shake-flask culture (0.0084 U/h). The present study provides valuable information about statistical optimization of bioprocess development for cost-effective production of laccase and other heterologous proteins inY. lipolyticafrom beet molasses as sole carbon source, thus allowing the valorization and decreasing environmental pollution of this agro-industrial waste.
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Researchers
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Claude Jolivalt (Fourth Researcher), Catherine Madzak (Third Researcher), (Second Researcher), Farshad Darvishi (First Researcher)
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