|
Title
|
Evaluation of the L-Asparaginase production in the yeast Yarrowia lipolytica
|
|
Type
|
Presentation
|
|
Keywords
|
L-Asparaginase, Yeast, Yarrowia lipolytica, Production, Anti-leukemia agent.
|
|
Abstract
|
L-Asparaginase (L-asparagine amidohydrolase EC 3. 5. 1. 1) catalyses the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also using in production of accrylamide free food. Due to the significant applications of this enzyme, the aim of this study was introduce a new microbial source for the production of eukaryotic L- Asparaginase. The production of L-Asparaginase was investigated in four strains of Yarrowia lipolytica by quality and quantity methods. The quality and quantity tests were done with rapid plate method in minimal asparagine agar medium and Nessler's reagent spectrophotometry method, respectively. The results showed that Y. lipolytica DSM3286 has the biggest halo in minimal asparagine agar medium and produced the highest enzyme activity in comparison with other strains. Therefore, yeast Y. lipolytica could be introduced as a new microbial and eukaryotic source for the production of L-Asparaginase.
|
|
Researchers
|
Fereshteh Shamsi (Second Researcher), Farshad Darvishi (First Researcher)
|