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Title
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Laccase production from sucrose by recombinant Yarrowia lipolytica
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Type
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Presentation
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Keywords
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Laccase, Yarrowia lipolytica, Trametes versicolor, Sucrose, Bioreactor.
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Abstract
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Background: Laccases are multi-copper enzymes that oxidized various aromatic and non-aromatic compounds and have diverse industrial applications from xenobiotic compounds degradation to biofuel production. The aim of this study was to investigate the effect of sucrose as a substrate on the laccase production by a recombinant strain of Yarrowia lipolytica in bioreactor. Methods: A batch culture of Y. lipolytica YL4 in 5 L bioreactor was carried out with 1.5 L of modified PPB medium containing 10% sucrose as a sole carbon source. The yeast cell growth was monitored by direct counting method on a haemocytometer. Laccase activity was determined spectrophotometrically by monitoring ABTS oxidation at 420 nm. Results: The laccase activity reached to 6.7 U/mL in bioreactor with modified PPB medium after 48 h which is 33.5 fold higher than PPB medium containing 2% glucose in the previous studies. Conclusion: Sucrose as a cost-effective and sole carbon source could be used to large scale production of laccase and other recombinant protein by Y. lipolytica.
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Researchers
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Claude Jolivalt (Fourth Researcher), Catherine Madzak (Third Researcher), Marzieh Moradi (First Researcher), Farshad Darvishi (Second Researcher)
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