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Abstract
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Background: Laccases have various environmental applications, such as textile dye bleaching, pulp delignification and bioremediation of wastewater. The aim of the study was evaluate the ability of laccase from recombinant Yarrowia lipolytica for decolorization of some environmental pollutant dyes. Methods: The supernatant of Yarrowia lipolytica YL4 culture containing laccase was used to decolorization of seven dyes Bromocresol purple, Safranin, Malachite green, Kristal violet, Bromothymol blue, Nigrosine and Phenol red. The maximum absorbance and decolorization of each dye was determined using microtiter plate reader. The effects of pH, concentration of enzyme and incubation time were investigated on decolorization. Results: The laccase of Y. lipolytica YL4 was able to decolorization of dyes. The effect of incubation time was important which decolorization efficiency was reached more than 90 percent after 48 hours. Conclusion: The laccase of Y. lipolytica was able to decolorization of environmental pollutant dyes that could be used to bioremediation of industrial wastewater.
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