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Abstract
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A new method based on CdSe quantum dots (QDs) was developed to detect 185delAG mutation in BRCA1 gene. In this method P2-NH2 strand was covalently attached to QDs possessing carboxylic acid to provide QD-P2, and its emission was studied against other probs. In the presence of cDNA and P1-NH2, emission of QD-P2 was 85% more intense than just pure bare QD-P2. Moreover, new peak appeared in emission spectrum in higher wavelength which could be associated with formation of more organized shrinked aggregates due to connection between QDs by DNA strands. Partial (35%) increase in the intensity of emission in the presence of mDNA was observed, according to the formation of disorganized aggregates and therefore less rigid system, which is attributed to mutation in DNA strands. The generation of organized small and disorganized aggregates in the presence of cDNA and mDNA, respectively, was approved by DLS analysis and zeta potential measurements.
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