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Title
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Interaction of Yarrowia lipolytica lipase with dithiocarbamate modified magnetic carbon Fe3O4@ C-NHCS2H core-shell nanoparticles
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Type
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JournalPaper
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Keywords
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Yarrowia lipolyticaLipase immobilizationBiocatalystFe3O4@CCore-shellDithiocarbamate
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Abstract
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Fe3O4@C core-shell nanoparticles were modified by (3-aminopropyl)triethoxysilane (APTES) to generate amine functionality in the surface. Then, the amine functional groups were converted to dithiocarbamate via post-modification with carbon disulfide. This nanostructure with new functional property was used to immobilize lipase (obtained from Yarrowia lipolytica U6). Biocatalytic activity of the Fe3O4@C-NHCS-LIP was studied in this project. The interaction of lipase and support though dithiocarbamate binder was examined in the hydrolysis of p-nitrophenyl laurate. In this paper, support showed a unique feature in the immobilization of lipase by maintaining the lipase activity, raising the stability of lipase, and reusability.
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Researchers
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Zahra Fathi (First Researcher), Yusuke Ide (Not In First Six Researchers), Ameneh Ghodsi (Fifth Researcher), Farshad Darvishi (Fourth Researcher), Sadegh Rostamnia (Third Researcher), Esmail Doustkhah (Second Researcher)
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