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Title
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Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells
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Type
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JournalPaper
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Keywords
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Layered double hydroxide; nanomaterial; drug/gene delivery; pCEP4/Cdk9
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Abstract
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Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for
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Researchers
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vahid yousefi (Fourth Researcher), Mir Mahdi Abolghasem (Not In First Six Researchers), Vahideh Tarhriz (Not In First Six Researchers), Asghar Mesbahi (Not In First Six Researchers), Mohammad Saeid Hejazi (Not In First Six Researchers), Homan Kahroba (Fifth Researcher), Shirin Eyvazi (Third Researcher), Elham Mansouri (Second Researcher), Parivar Yazdani (First Researcher)
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