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Title
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Noncovalent immobilization of Yarrowia lipolytica lipase on Dendritic-like amino acid-functionalized silica nanoparticles
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Type
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JournalPaper
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Keywords
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Yarrowia lipolytica; lipase; biocatalysis; immobilization; dendrimer
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Abstract
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Immobilization of enzymes is a promising approach for the cost-e ective application of enzymes. Among others, noncovalent but unleachable approaches for immobilization are one of the most favorable and crucial approaches. Herein, silica nanoparticles are modified by (3-aminopropyl)triethoxysilane (APTES) to generate amino-functionalized silica nanoparticles. Then, the amine functionalities are converted to bifunctional amino acid via post-modification that has zwitterionic properties. This nanostructure with the new functional theme is employed to immobilize Yarrowia lipolytica lipase at room temperature with no further post-modification or cross-linking. This immobilization method is further compared with the metal chelate-based immobilization approach on the same support. The biocatalytic activity of the immobilized lipase is examined under various conditions. The encapsulation of lipase through amino acid-functionalized silica nanoparticles exhibited enhanced stability for the immobilized lipase at higher temperatures and unneutral pHs.
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Researchers
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Farshad Darvishi (Fourth Researcher), (Third Researcher), (Second Researcher), Zahra Fathi (First Researcher)
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