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Title
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Kappa-Carrageenan Crosslinked Magnetic Folic Acid-Conjugated Chitosan Nanocomposites for Arginase Encapsulation, Delivery and Cancer Therapy
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Type
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JournalPaper
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Keywords
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Pichia pastoris; chitosan nanocomposites; L-arginase; drug delivery; cancer therapy
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Abstract
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In this study, the Aspergillus niger L-arginase was expressed in Pichia pastorise and immobilized on Kappa-carrageenan crosslinked magnetic folic acid-conjugated chitosan nanoparticles (magnetic-FA/CTS NPs). L-arginase gene was isolated and cloned in pGAPZA plasmid and transformed in P. pastoris. The puri¯ed L-arginase was loaded on magnetic-FA/CTS NPs nanocomposites and the structure of the synthesized nano-carriers was investigated using SEM, FT-IR and DLS techniques. Prepared formulations showed high encapsulation e±ciency (91.93%). In vitro release and cytotoxic studies were performed at di®erent pH (pH of 4–8) and against Molt-4 cancer cell line, respectively.
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Researchers
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Ali Akbari (Third Researcher), Reza Mohammadzadeh (Second Researcher), Rahman Moradi (First Researcher)
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