Catharanthus roseus is containing anticancer alkaloids of vincristine and vinblastine and is an important medicinal plant. Several studies have conducted on in‐vitro culture of this plant. To optimize the somatic embryogenesis, a factorial based on CRD experiment with 10 replications was conducted. Root, hypocotyl and leaf explants grown invitro were transferred and cultured on MS media containing different combinations of 2,4D, NAA and 2,4D×BAP. The results revealed that in callogenesis, the interaction effects of root and hypocotyl explants×2,4D and NAA as well as hypocotyl×(1 mg l1 NAA+1 mg l1 BAP) was superior than other treatments (p≤0.01). For calli fresh weight, hypocotyl×NAA and hypocotyl× (1 mg l1 NAA+1 mg l1 BAP) was the treatment of choice (p≤0.01). The calli produced were subcultured to attain the preembryos and somatic embryos. For the number of preembryos and somatic embryos; the interaction of hypocotyl×2,4D was the most efficient treatment. Seemingly, the production of somatic embryos is accessible in this plant by the logical management of growth regulator combinations. Furthermore, the production and genetic engineering of the somatic embryos could be a promising trend in the subsequent production of highvalued metabolites from this plant.
