عنوان مجله
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DARU-Journal of Pharmaceutical Sciences
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کلیدواژهها
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Pistacia atlantica, Breast carcinoma, Cell cycle, Cyclins, Doxorubicin
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چکیده
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Background and the purpose of the study: Natural products from plants have an important role in the
development and production of new drugs mainly for cancer therapy. More recently, we have shown that the
pericarp methanolic extract of Pistacia atlantica sub kurdica (with local name of Baneh) as a rich source of active
biological components with high antioxidant and radical scavenging activities, has ability to cease proliferation and
induce apoptosis in T47D human breast cancer cells. The present study aimed to clarify whether Baneh extract able
to alter cell cycle progression of T47D cells or not.
Methods: In order to study the possible effect of Baneh extract on cell cycle of T47D cells, we evaluated cell cycle
distribution and its regulatory proteins by flow cytometry and western blot analysis respectively.
Results: Baneh extract induced G0/G1 cell cycle arrest in conjunction with a marked decrease in expression of cyclin
D1 and cdk4 that was strongly dependent on time of exposure. In parallel, Dox-treated T47D cells in early time
points were accumulated on S phase, but after 48 h cell cycle progression was inhibited on G2/M. Dox promoted
striking accumulation of cyclin B1 rapidly and enhanced cyclin A abundance.
Conclusion: Taken together, our results establish that the antitumor activity of the pericarp extract of Baneh partly
is mediated via cell cycle arrest and downregulation of cyclin D1 and cdk4 expression. These findings warrant
further evaluation regarding the mechanism(s) of action of this promising anticancer agent.
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