چکیده
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Abstract It is now widely accepted that dietary
phytochemicals inhibit cancer progression and
enhance the effects of conventional chemotherapy.
In this report, we comparatively studied the cellular
and molecular aspects of apoptosis induction by the
methanolic extract of Baneh fruit skin in comparison
to Doxorubicin (Dox), a well-known anticancer
drug, in human breast cancer T47D cells. The
MTT assay was used to determine the antiprolifer-
ative effects. The flow cytometric and microscopic
analyses were done to evaluate the apoptosis induc-
tion. Furthermore, western blot analyses have been
done to study the role of key molecular players of
apoptosis including caspase 3 and PARP. The Baneh
extract showed strong antiproliferative activity against T47D cells in a dose- and time-dependent
manner that was comparable to and even stronger
than Dox in certain concentrations. Analysis of
Baneh-treated cells by flow cytometry and fluores-
cence microscopy indicated strong apoptosis induc-
tion and nuclear morphological alterations similar to
or greater than Dox. Finally, molecular analysis of
apoptosis by western blotting proved activation of
caspase 3 followed by poly ADP ribose polymerase
(PARP) cleavage more efficiently in Baneh than in
Dox treated cancer cells. These findings indicate that
Baneh extract contains phytochemicals which act as
inhibitor of cell proliferation and inducer of apop-
tosis in human breast cancer T47D cells that makes
it a potentially good candidate for new anticancer
drug development.
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