چکیده
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Amethod was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible
for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The
protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye
(FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA.
In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5
terminal in the presence of cDNA or mDNA was applied.
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