عنوان مجله
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Applied Biochemistry and Biotechnology
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چکیده
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Laccases are used in numerous applications, from green degradation of various
xenobiotic compounds, waste detoxification, textile dye bleaching, and delignification of
lignocellulose materials to biofuel production. In this study, the recombinant Yarrowia
lipolytica YL4 strain carrying the white-rot fungusTrametes versicolorlaccaseIIIbgenewas
used for laccase production from beet molasses as an agro-industrial residue. Response surface
methodology was used to statistical optimization of the production of laccase byY. lipolytica
using an industrial medium containing molasses which allows a six times increase in laccase
activity compared to primary medium contains glucose after 144 h. In bioreactor cultivation
after 48 h, laccase production reached to 3.7- and 22.5-fold more than optimized and primary
media in shake-flask cultures, respectively. Laccase productivity in bioreactor (0.0937 U/h) was
higher than shake-flask culture (0.0084 U/h). The present study provides valuable information
about statistical optimization of bioprocess development for cost-effective production of laccase
and other heterologous proteins inY. lipolyticafrom beet molasses as sole carbon source, thus
allowing the valorization and decreasing environmental pollution of this agro-industrial waste.
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