مشخصات پژوهش

: The cabbage cyst nematode, Heterodera cruciferae Franklin, 1945 is a sedentary plant parasitic nematode which in comparison to related species, has gained less attention from a part of researchers due to its limited geographical distribution and relatively lower crop losses. The nematode has been reported from England, United States of America, Turkey, Russia and Iran. All members of Cruciferaceae as well as Lamiaceae are infected by the nematode. Heterodera cruciferae infects different species of plants in the vegetable growing areas of Tabriz, northwestern Iran. The parasitism of plant nematodes is in the close relation with expression of some genes beside some differentiations in morphology (stylet), behavior, reproduction and survival strategies in nematode. In plant parasitic nematodes the effectors can originated and secreted from esophageal glands, amphid and cuticle which the first one is the most important and main region to these products. In order to study the nematode post infective stage transcriptome, different life stages of the nematode including J3, J4 and adults were dissected from host plants, kohlrabi (Brassica oleracea L. var. gongylodes) and white cabbage (Brassica oleraceaL.var.captita alba) roots using fine needles. The Nucleospin®Triprep kit, mini prep, Machery-Nagel-Germany kit used for RNA extraction and the quality of the extracted RNAs was tested by RNA Nano Chip-USA, Agilent RNA6000 Nano Kit-USA and Bioanalzyer Agilent 2100 –USA. All extracted RNAs with more than seven RIN (RNA integrity) factor were sent to BGI TECH SOLUTIONS Hong-Kong, China for preparation of the nematode transcriptome using Next generation sequencing- (Illumina) method. The transcriptome analysis of the studied Iranian population of H. cruciferae revealed that most of the known parasitism genes from other plant parasitic nematodes such as potato cyst nematode, sugar beet cyst nematode, reniform nematode and pine wood nematode were also expressed in cabbage cyst ne