عنوان مجله
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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چکیده
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Chitosan-based magnetic beads were developed by solution-mixing method. Firstly, the Fe3O4 nanoparticles were in situ immobilized on laponite RD sheets. The magnetic laponite RD was then dispersed in
PVA and mixed with chitosan solution. PVA was aimed to prevent the disintegration of chitosan under
acidic media due to its ability to form hydrogel network through freezing-thawing method. The manufactured magnetic chitosan/PVA/laponite RD beads were utilized for adsorption study of a model protein,
bovine serum albumin (BSA). The adsorption of BSA on beads was pH-dependent where smaller mass
of protein was adsorbed at pH values lower than isoelectric point of BSA. Moreover, it was discovered
that introduction of magnetic laponite RD can improve the adsorption capacity of magnetic beads for
BSA in which hydrogel with the highest content of magnetic laponite RD demonstrated the maximum
adsorption capacity for BSA (qm = 240.5 mg/g). Langmuir model described the isotherm data better than
Freundlich model.
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