مشخصات پژوهش

Signal peptide (SP) is an important factor and biobrick in the production and secretion of ‎recombinant proteins. The aim of this study was in silico and in vivo analysis of SPs effect on ‎the production of recombinant glucose oxidase (GOX) in Yarrowia lipolytica. Several in silico ‎softwares, namely SignalP4, Signal-CF, Phobius, WolfPsort 0.2, SOLpro and ProtParam, were ‎used to analyse the potential of fifteen endogenous and exogenous SPs for the secretion of ‎recombinant GOX in Y. lipolytica. According to in silico results, the SP of GOX was predicted ‎as suitable in terms of high secretory potential and of protein solubility and stability which is ‎chosen for in vivo analysis. The recombinant Y. lipolytica strain produced 280 U/L of ‎extracellular GOX after 7 days in YPD medium. The results show that the SP of GOX can be ‎applied to efficient production of extracellular heterologous proteins and metabolic engineering ‎in Y. lipolytica.‎