مشخصات پژوهش

The purpose of this study was to investigate, the effect of static magnetic field on OCT4 gene expression in rat bone marrow mesenchymal stem cells (rBMSC). The rBMSCs are multipotent stem cells that can differentiate to other cells such as neural cells. This issue can be useful in treating nerve injured tissue. rBMSCs were cultured after extraction from rat femur bone. These cells were treated with Neural inductive medium (NIM) concomitantly with 15 and 30 mT static magnetic field (SMF). The control groups were not exposed to SMF. Samples were exposed to NIM and SMF 2,4 and 6 hours. RNA molecules were extracted and were used as a cDNA template. cDNA s were amplified in q-RT-PCR. The threshold cycle ( Ct) was compared in target gene (OCT4) and internal control gene (GAPDH). the OCT4 expression level was calculated in target and control group according to reference gene expression level. The data was analyzed using SPSS 19 software. Our results shows that, the OCT4 expression was decreased in three different time exposures against the 15 and 30 mT, SMF (p<0.01). It seems that two applied intensities of SMF, reduced the level of OCT4 expression significantly. OCT4 is required to self renewing and maintaining in stemness state. Our results shows that SMF in different intensity, 15 and 30 mT, reduced the OCT4 expression in three different time of Exposure 2, 4 and 6 hours. This means SMFs help the rBMSCs to leave their stemness position and under go to